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Sterilization of the biological safety cabinet

机译:生物安全柜灭菌

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摘要

Test strips comprising B subtilis spores or M phlei cells dried on aluminium foil (some serum-coated) were used to test sterilizing processes currently advised for safety cabinets. Exposure to formalin from a bowl, boiling excess formalin with the fan running, and ultraviolet radiation were all found to be unreliable, as was glutaraldehyde. Vaporization of 2 ml of formalin per 0·028 m3 followed by exposure for 18 hours at ambient temperatures was 100% successful, and the depolymerization of paraformaldehyde tablets with a hair drier was a simple, very effective alternative method.
机译:包含枯草芽孢杆菌B或M phlei细胞的测试条在铝箔上干燥(涂有一些血清),用于测试目前建议用于安全柜的灭菌过程。发现从碗中暴露于福尔马林,在风扇运转下沸腾过量的福尔马林,以及紫外线辐射都是不可靠的,戊二醛也是如此。每0·028 m3汽化2 ml福尔马林,然后在环境温度下暴露18小时是100%成功的,用吹风机将多聚甲醛片剂解聚是一种简单,非常有效的替代方法。

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